Background:  CNK1 (connector enhancer of kinase suppressor of Ras 1), also known as KSR or CNKSR1, is a 720 amino acid cytoplasmic and membrane protein involved in the RAS-dependent signaling pathway. RAS is essential for integrating and transmitting proliferation, differentiation and survival signals elicited by membrane receptors to downstream effector pathways. RAF is part of the RAS dependent signaling pathway and is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. CNK1 contains several protein-protein interaction domains and plays a key role as a tyrosine phosphorylation target in multiple receptor tyrosine kinase pathways. The C-terminal portion of CNK1 directly binds to RAF, blocking RAS- and RAF-dependent signaling when overexpressed. The N-terminal portion contains two domains that are critical for cooperation with RAS, and two CNK1 isoforms are produced due to alternative splicing events.

Description: Rabbit polyclonal to CNKSR1

Immunogen: KLH conjugated synthetic peptide derived from CNKSR1

Specificity:  ·Reacts with Human, Mouse, Pig, Dog and Rat.

·Isotype: IgG

Application:  ·Western blotting: 1/100-500. Predicted Mol wt: 80 kDa;

·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;

·Immunocytochemistry/Immunofluorescence: 1/100;

·Immunoprecipitation: 1/50;

·ELISA: 1/500;

·Optimal working dilutions must be determined by the end user.