Background:  The plasma membrane Ca2+-pumping ATPase (PMCA) mRNAs are encoded on four genes designated PMCA1-4. The PMCA genes undergo alternative splicing to yield “b” splice forms, which contain PDZ interaction domains and interact promiscuously but also specifically with different members of the PSD95 family. PMCA4b is the major PMCA expressed in developing mammary tissue. During lactation, PMCA1b expression increases while PMCA4b expression decreases, indicating that PMCAs play a critical role in maintaining cellular Ca2+ homeostasis. In addition, human PMCA4b may have an important role in regulating intracellular Ca2+ in the apoptotic cell. PMCA4b is cleaved at Asp 1080 by caspase-3 to produce a single fragment that is fully active, responding much faster to an increase in Ca2+ than the autoinhibited form. PMCA4b also plays an essential role in maintaining low cytosolic Ca2+ in resting platelets. Specifically, PMCA4b is phosphorylated on Tyr 1176 by pp60 (src).

Description: Rabbit polyclonal to PMCA4B

Immunogen: KLH conjugated synthetic peptide derived from PMCA4B

Specificity:  ·Reacts with Human, Mouse and Rat.

·Isotype: IgG

Application:  ·Western blotting: 1/100-500. Predicted Mol wt: 130 kDa;

·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;

·Immunocytochemistry/Immunofluorescence: 1/100;

·Immunoprecipitation: 1/50;

·ELISA: 1/500;

·Optimal working dilutions must be determined by the end user.