Background:  Human thiol dioxygenases include ADO (2-aminoethanethiol (cysteamine) dioxygenase) and CDO (cysteine dioxygenase). ADO adds two oxygen atoms to free cysteamine to form hypotaurine, whereas CDO adds two oxygen atoms to free cysteine. Encoded by a gene that maps to human chromosome 10q21.2, ADO is a 270 amino acid protein that is ubiquitously expressed, with highest levels in brain, heart and skeletal muscle. ADO is responsible for endogenous cysteamine dioxygenase activity and participates in metal ion binding, with iron as a cofactor. Overexpression of ADO in HepG2/C3A cells increases production of hypotaurine from cysteamine. Conversely, reduced expression of ADO decreases hypotaurine production.

Description: Rabbit polyclonal to ADO

Immunogen: KLH conjugated synthetic peptide derived from ADO

Specificity:  ·Reacts with Human, Mouse, Dog and Rat.

·Isotype: IgG

Application:  ·Western blotting: 1/100-500. Predicted Mol wt: 30 kDa;

·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;

·Immunocytochemistry/Immunofluorescence: 1/100;

·Immunoprecipitation: 1/50;

·ELISA: 1/500;

·Optimal working dilutions must be determined by the end user.