Background:  IQGAP1 and IQGAP2 are RasGAP-related Actin binding proteins that interact with the small GTPases Cdc42 and Rac1 and regulate cadherin-mediated cell-cell adhesion. IQGAP1 and IQGAP2 share largely related sequence similarity, and both contain a putative calponin domain, a single WW domain, four conserved IQ or calmodulin-binding domains, and a RasGAP domain. IQGAP1 binds preferentially to the GTP S-bound form of Cdc42, whereas IQGAP2 associates with both nucleotide-bound and nucleotide-free forms of Cdc42. In addition to binding Cdc42, IQGAP1 and IQGAP2 also bind Rac1, F-Actin and calmodulin. The binding of IQGAP proteins to Cdc42 and Rac1 inhibits their intrinsic and RhoGAP-stimulated GTPase activities, which thereby maintains Cdc42 and Rac1 in their active GTP-bound state.

Description: Rabbit polyclonal to IQGAP2

Immunogen: KLH conjugated synthetic peptide derived from IQGAP2

Specificity:  ·Reacts with Human, Mouse and Rat.

·Isotype: IgG

Application:  ·Western blotting: 1/100-500. Predicted Mol wt: 181 kDa;

·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;

·Immunocytochemistry/Immunofluorescence: 1/100;

·Immunoprecipitation: 1/50;

·ELISA: 1/500;

·Optimal working dilutions must be determined by the end user.