Background:  Belonging to the sterol desaturase family, ERG25, also known as C-4 methylsterol oxidase or Methylsterol monooxygenase, is a 293 amino acid enzyme that performs the first of three enzymatic steps required to remove the two methyl groups leading to cholesterol. ERG25 is a multi-pass membrane protein that resides within the plasma membrane and the membrane of the endoplasmic reticulum. Concentrations of ERG25 mRNA increase when subjected to low iron growth conditions, suggesting that the enzyme is not regulated by iron but by an end product of the ergosterol pathway. Low-density lipoprotein (LDL) downregulates ERG25 expression in the vascular wall, an event that may be inhibited via SREBP overexpression. ERG25 is encoded by a gene located on human chromosome 4q32.3.

Description: Rabbit polyclonal to ERG25

Immunogen: KLH conjugated synthetic peptide derived from ERG25

Specificity:  ·Reacts with Human, Mouse and Rat.

·Isotype: IgG

Application:  ·Western blotting: 1/100-500. Predicted Mol wt: 35 kDa;

·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;

·Immunocytochemistry/Immunofluorescence: 1/100;

·Immunoprecipitation: 1/50;

·ELISA: 1/500;

·Optimal working dilutions must be determined by the end user.