Background:  The Gab family of adaptor proteins function as molecular scaffolds that mediate protein recrutiment to RTKs. Cytokine/growth factor triggering of protein tyrosine kinase receptors (RTKs) initiates signaling cascades that progress to the nucleus where signals for activation, proliferation and differentiation occur. This scaffolding mechanism represents a critical link in cytokine/growth factor signaling routes. Gab1-3 contain pleckstrin homology and potential binding sites for SH2 and SH3 domain-containing proteins. The recruitment of signaling partners to Gab family members is phosphorylation dependent. Insulin receptor and EGF-receptor signaling are among the cascades that rely on Gab family members to elicit a nuclear response to an extracellular stimulus. The human Gab3 gene maps to chromosome Xq28 and encodes a 586 amino acid protein.

Description: Rabbit polyclonal to GAB3

Immunogen: KLH conjugated synthetic peptide derived from GAB3

Specificity:  ·Reacts with Human, Mouse, Pig, Dog and Rat.

·Isotype: IgG

Application:  ·Western blotting: 1/100-500. Predicted Mol wt: 66 kDa;

·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;

·Immunocytochemistry/Immunofluorescence: 1/100;

·Immunoprecipitation: 1/50;

·ELISA: 1/500;

·Optimal working dilutions must be determined by the end user.