Background:  Filaggrins are an important class of the intermediate filament-associated proteins which interect with keratin intermediate filaments (Ifs) of terminally differentiating mammalian epidermis. Filaggrins exhibit wide species variations. Filaggrin is expressed only in well-differentiated keratinized epithelial cells. The synthesis of filaggrin varies in types of ichthyosis, and with viral, premalignant and malignant conditions. Filaggrin also appears to be a target molecule for rheumatoid arthritis-specific auto-antibodies in humans. Keratohyalin granules in the granular layer of interfollicular epidermis are predominantly composed of the 400-kD protein profilaggrin. Following a short, unique N-terminal domain, most of the profilaggrin molecule consists of 10 to 12 repeats of the 324-residue filaggrin sequence. Upon terminal differentiation of granular cells, profilaggrin is proteolytically cleaved into filaggrin peptides of approximately 37 kD and the N-terminal domain containing an S100-like calcium-binding domain.

Description: Rabbit polyclonal to Filaggrin

Immunogen: KLH conjugated synthetic peptide derived from Filaggrin

Specificity:  ·Reacts with Human, Mouse, Pig, Dog and Rat.

·Isotype: IgG

Application:  ·Western blotting: 1/100-500. Predicted Mol wt: 40 kDa;

·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;

·Immunocytochemistry/Immunofluorescence: 1/100;

·Immunoprecipitation: 1/50;

·ELISA: 1/500;

·Optimal working dilutions must be determined by the end user.