Background:  HERC1 is a stimulates guanine nucleotide exchange on ARF1 and Rab proteins. This protein may be involved in membrane transport processes. One of the largest human proteins, Herc1, also designated p532 or p619, is a 4861 amino acid protein that contains a variety of functional domains, including WD repeats, RCC1 repeats, beta-repeat domains and one HECT domain. Herc1 is ubiquitously expressed in human and mouse tissues and is overexpressed in several human tumor cell lines. It localizes to the cytosol and the Golgi apparatus, where it acts as a guanine nucleotide exchange factor on ARF1 and Rab proteins to mediate membrane trafficking. Herc1 also is involved in proliferation and growth through its interactions with clathrin, M2-pyruvate kinase and TSC2 proteins. Mutations in the TSC2 protein allow for binding to Herc1 in the presence of TSC1, which destabilizes the TSC2 protein. This results in tuberous sclerosis complex (TSC), an autosomal dominant disease characterized by hamartoma formation in various organs.

Description: Rabbit polyclonal to HERC1

Immunogen: KLH conjugated synthetic peptide derived from HERC1

Specificity:  ·Reacts with Human, Mouse and Rat.

·Isotype: IgG

Application:  ·Western blotting: 1/100-500. Predicted Mol wt: 532 kDa;

·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;

·Immunocytochemistry/Immunofluorescence: 1/100;

·ELISA: 1/500;

·Optimal working dilutions must be determined by the end user.