Background:  M-RIP is a 1,025 amino acid cytoplasmic and cytoskeletal protein that is required for regulation of the actin cytoskeleton. M-RIP colocalizes with myosin binding subunit (MBS) to regulate the phosphorylation of myosin light chain, and colocalizes with F-actin through its N-terminus in the cytoskeleton. M-RIP also interacts with and RhoA at actin stress fibers via its adjacent coiled coil domains. M-RIP is highly expressed in ovary, with moderate levels found in brain, heart, liver, lung, skeletal muscle, testis and kidney. M-RIP depletion causes an increase of stress fibers in smooth muscle cells, whereas M-RIP over-expression causes disassembly of stress fibers in neuronal cells. Containing two PH domains, M-RIP has multiple phosphorylated serine and threonine residues and exists as three isoforms which are produced by alternative splicing events.

Description: Rabbit polyclonal to MPRIP

Immunogen: KLH conjugated synthetic peptide derived from MPRIP

Specificity:  ·Reacts with Human, Mouse and Rat.

·Isotype: IgG

Application:  ·Western blotting: 1/100-500. Predicted Mol wt: 117 kDa;

·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;

·Immunocytochemistry/Immunofluorescence: 1/100;

·Immunoprecipitation: 1/50;

·ELISA: 1/500;

·Optimal working dilutions must be determined by the end user.