Background:  The RING finger motif is a specialized DNA-binding zinc finger domain found in many transcriptional regulatory proteins. The RING finger protein (RNF) family includes any protein containing the signature RING finger motif. RNF8 is an ubiquitously expressed, nuclear RING finger protein that acts as an E3 ubiquitin-protein ligase. It is required for the ubiquitination of some nuclear proteins and promotes their subsequent degradation. The heterodimeric ubiquitin-conjugating enzyme UBC13 interacts with RNF8, and they co-localize in the nucleus. RNF8 may regulate mediation of UBC13 polyubiquitylation by elongating the ubiquitin chains. RNF8 also binds to Retinoid X receptor α (RXRα), a member of the steroid hormone receptor superfamily. It increases RXRα-mediated transactivation of the RXRα-responsive element (RXRE) promoter in a dose-dependent manner, suggesting that RNF8 is a regulator of RXRα-mediated transcriptional activity.

Description: Rabbit polyclonal to RNF8

Immunogen: KLH conjugated synthetic peptide derived from RNF8

Specificity:  ·Reacts with Human, Mouse, Pig, Dog and Rat.

·Isotype: IgG

Application:  ·Western blotting: 1/100-500. Predicted Mol wt: 55 kDa;

·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;

·Immunocytochemistry/Immunofluorescence: 1/100;

·Immunoprecipitation: 1/50;

·ELISA: 1/500;

·Optimal working dilutions must be determined by the end user.