Background:  The deduced 198 amino acid MAP kinase phosphatase 6 (MKP-6), also designated MAP6 and dual-specificity phosphatase 14 (DUSP14), is homologous to other MKP family proteins in that it has a conserved, centrally located, catalytic core, but differs from traditional MKP proteins because it contains unique N- and C-terminal regions. Binding and deletion analyses have established that the interaction between the cytoplasmic tail of CD28 (a T cell antigen) and MKP-6 occurs at Tyr 200 of CD28 and is specific for both MKP-6 and CD28; however, Tyr 200 can be mutated to Phe 200 without a loss of binding ability. Functional analysis indicates that MKP-6 dephosphorylates ERK, JNK and p38 while acting as a negative regulator of CD28 signaling. MKP-6 is expressed ubiquitously, although expression is stronger in certain cell types and tissues than in others.

Description: Rabbit polyclonal to DUSP14

Immunogen: KLH conjugated synthetic peptide derived from DUSP14

Specificity:  ·Reacts with Human, Mouse and Rat.

·Isotype: IgG

Application:  ·Western blotting: 1/100-500. Predicted Mol wt: 22 kDa;

·Immunohistochemistry (Paraffin/frozen tissue section): 1/100-500;

·Immunocytochemistry: 1/100;

·Immunoprecipitation: 1/50;

·ELISA: 1/500;

·Optimal working dilutions must be determined by the end user.