Background:  Golgin 84 is a ubiquitously expressed single-pass type II coiled-coil membrane protein. Localizing to the Golgi apparatus and predominantly found on membranes at the cis side of the Golgi stack, golgin 84 participates in the maintenance of Golgi structure and the formation of Golgi stacks and ribbons. The depletion of golgin 84 leads to fragmentation of the Golgi ribbon and reduced efficiency in protein transport. In addition, golgin 84 binds to active Rab 1 and associates with CASP (an isoform of CDP) in a golgin-tethering complex that is believed to play a role in intra-Golgi retrograde transport. During mitosis, golgin 84 is highly phosphorylated. Chromosomal translocations involving the gene encoding golgin 84 have been associated with cancer tissues. The chimeric proteins produced by this translocation are known as Ret-II and PTC5.

Description: Rabbit polyclonal to GOLGA5

Immunogen: KLH conjugated synthetic peptide derived from GOLGA5

Specificity:  ·Reacts with Human, Mouse and Rat.

·Isotype: IgG

Application:  ·Western blotting: 1/100-500. Predicted Mol wt: 83 kDa;

·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;

·Immunocytochemistry/Immunofluorescence: 1/100;

·Immunoprecipitation: 1/50;

·ELISA: 1/500;

·Optimal working dilutions must be determined by the end user.